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Fig. 4 | BMC Microbiology

Fig. 4

From: Molecular cloning and characterization of vanillin dehydrogenase from Streptomyces sp. NL15-2K

Fig. 4

Conversion of vanillin to vanillic acid by VDH. HPLC analysis was used to examine vanillin conversion to vanillic acid by VDH activity in cell-free extracts. A cell-free extract of E. coli harboring pET-28a (a) or pET28a/VDH (b) was incubated with 1.25 mM vanillin in the presence of 2 mM NAD+ for 10 min. HPLC analysis was conducted according to the conditions described in Methods. E. coli strains were cultured for 15 h after IPTG induction

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