Fig. 4
From: Molecular cloning and characterization of vanillin dehydrogenase from Streptomyces sp. NL15-2K
Conversion of vanillin to vanillic acid by VDH. HPLC analysis was used to examine vanillin conversion to vanillic acid by VDH activity in cell-free extracts. A cell-free extract of E. coli harboring pET-28a (a) or pET28a/VDH (b) was incubated with 1.25Â mM vanillin in the presence of 2Â mM NAD+ for 10Â min. HPLC analysis was conducted according to the conditions described in Methods. E. coli strains were cultured for 15Â h after IPTG induction