Fig. 2
From: The culture of primary duck endothelial cells for the study of avian influenza
Sorting Ac-LDLlo cells can be used to isolate a pure population of duck endothelial cells. a&b Representative FACS plots of chicken bone marrow-derived cells following 15 days of culture in human endothelial cell medium. Cells were incubated with Alexa Flour®488 conjugated Ac-LDL for 4 h and then stained for anti-chicken CD45. Single cells (as defined by FCS-A/SSC-A) were assessed for the expression of CD45 and uptake of Ac-LDL. c Representative FACS plot of sorted Ac-LDLlo chicken bone marrow-derived cells. Cells were cultured for one passage in human endothelial cell medium, incubated with Alexa Flour®488 conjugated Ac-LDL for 4 h and then stained for anti-chicken CD45. Single cells (as defined by FCS-A/SSC-A) were assessed for the expression of CD45 and uptake of Ac-LDL. d RT-PCR for vWF expression on sorted Ac-LDLlo chicken bone marrow-derived cells. ‘No RT’ = samples where RNA was used as the template. e Representative FACS plot of sorting strategy used on duck bone marrow-derived single cells following 15 days of culture in human endothelial cell medium. Cells were incubated with Alexa Flour®488 conjugated Ac-LDL for 4 h and then stained for anti-chicken CD45. f&g RT-PCR for vWF (f) and CD45 (g) expression on sorted Ac-LDLlo duck bone marrow-derived cells. ‘No RT’ = samples where RNA was used as the template