Fig. 3From: Dickeya zeae strains isolated from rice, banana and clivia rot plants show great virulence differentialsThe number of bacterial cells of all the tested D. zeae strains invading into potato (a), cabbage (b), banana (c) and clivia (d), respectively. Healthy plant materials were surface-sterilized and inoculated with 2 μL of bacterial overnight cultures (OD600 = 2.0) in LB medium, and incubated at 28 °C. Tissues were taken out after 12 h and 24 h, respectively. And the diseased and surrounding healthy tissues in equal weight were cut and ground, and then added with 10 mL of sterilized 0.85% NaCl solution, stirred evenly, and 1 mL of which was diluted in series gradients, and 100 μL in each gradient was spread evenly onto LB agar plates in triplicates and kept at 28 °C for 24 h. Colonies between 30 to 300 CFU were counted. LB medium was used as a negative control. Each assay was repeated three times with duplicatesBack to article page