Table 2 Sequences of the primers used in synthesis of dsRNAs and for expression analysis by RT-qPCR
Target gene | Primer name | Primer sequence (nt) – Primers are listed 5′- to − 3′ | Reference |
|---|---|---|---|
A Primers for dsRNAs synthesis | |||
 Argonaute 2 | AGO-2 T7-F | TAATACGACTCACTATAGGGGTCTTAGCATCCAACAACCA | This study |
AGO-2Â T7-R | TAATACGACTCACTATAGGGTGTCTATGCCGCACTCTTTC | ||
 Tsetse EP | TseEPT7-F | TAATACGACTCACTATAGGGCTACGATAAATATGTCCCTC​TAAT | Modified from [45] |
TseEPT7-F | TAATACGACTCACTATAGGGATCGGGCAAACCCTCAAC | ||
A Primers for q-RT-PCR | |||
 Argonaute 1 | AGO-1qPCR-F | CAACTGCTCGTTCGGCTCCA | This study |
AGO-1qPCR-R | GGCAAAACTCGTCCTCTTACTTCCA | ||
 Argonaute 2 | AGO-2qPCR-F | CGTTGGATGATGGCACAAAGATG | This study |
AGO-2qPCR-R | GCTGCCTGATGTGATGCAATTC | ||
 Argonaute 3 | AGO-3qPCR-F | GCACAACTAGCAGAGATGACAGATAC | This study |
AGO-3qPCR-R | TGCAGGGCAATCTTTTGGACAAT | ||
 Dicer 2 | DCR-2qPCR-F | GTAGAGCGAAGATACACGGCTAAA | This study |
DCR-2qPCR-R | CACCATAAATTGCGGCCTAATGAC | ||
 Drosha | DroshaqPCR-F | TCAAAACCAAGGACAGAGCGGA | This study |
DroshaqPCR-R | GCAAACGGGGAAAAAGGCAAAC | ||
 Tsetse EP | TseEPqPCR-F | ACCGTTCGTTCGCTTTACTAC | Modified from [45] |
TseEPqPCR-R | ACCAGCAGCCGTTTGACTTTC | ||
 GpSGHV (odv-e66) | GpSGHVqPCR-F | CAAATGATCCGTCGTGGTAGAA | [3] |
GpSGHVqPCR-F | AAGCCGATTATGTCATGGAAGG | ||
 GpSGHV (Maltodextrin glycosyltransferase, tegument protein) | GpSGHV32F | ACGCTGAACTAAATTATCGTCATCTACACG | This study |
GpSGHV31R | CACAGAATCGTCATCATCATCATCTACAGA | ||
 GpSGHV (capsid protein) | GpSGHV92F | TATATTGTAATCCACGACCGGAAACTGAAC | This study |
GpSGHV91R | TCGGTAGGCGTGAATGAACGTTTT | ||
 β-Tubulin (tsetse) | Tse-TubqPCR-F | GATGGTCAAGTGCGATCCT | [66] |
Tse-TubqPCR-R | TGAGAACTCGCCTTCTTCC | ||