Fig. 5
Validation of knockdown of AGO2 and tsetse EP (control) genes in G. pallidipes. RT-qPCR expression analysis of: a) AGO2; and b) tsetse EP post PBS/virus injection, following AGO2 and tsetse EP dsRNAs injection, respectively, compared to water injected flies (negative controls). Gene expression values were normalized to β–tubulin and transformed by the Box-Cox process (Expressionλ - 1)/λ). Regression lines marked with the same lower-case letter do not differ at the 0.05 level