Nuclear and Wolbachia-based multimarker approach for the rapid and accurate identification of tsetse species

BMC Microbiology

Table 2 A list of the molecular markers and primers used in this study

Molecular marker	Marker		Primer name	Primer sequence 5’-3’	Reference	Method of analysis
Nuclear markers	ITS1		GlossinaITS1_for	GTGATCCACCGCTTAGAGTGA	(Dyer et al., 2008) [16]	Gel electrophoresis
	ITS1		GlossinaITS1_rev	GCAAAAGTTGACCGAACTTGA
	Microsatellite markers	A10	A10 F	GCAACGCCAAGTGAAATAAAG
		A10	A10 R	TACTGGGCTCGCGTACATAAT
		Gmm14	Gmm14 F	CACACCCTGGATTACAAA	(Baker & Krafsur, 2001) [19]
		Gmm14	Gmm14 R	TGAAATGCAACCCTTCTT	(Baker & Krafsur, 2001) [19]
Mitochondrial markers	COI		COI	TTGATTTTTTGGTCATCCAGAAGT	(Dyer et al., 2008) [16]	Sequencing
	COI		CULR	TGAAGCTTAAATTCATTGCACTAATC
	16S rRNA		NI-J-12585	GGTCCCTTACGAATTTGAATATATCCT
	16S rRNA		LR-N-12866	ACATGATCTGAGTTCAAACCGG
	12S rRNA		12SCFR	GAGAGTGACGGGCGATATGT	(Doudoumis et al., 2012) [52]
	12S rRNA		12SCRR	AAACCAGGATTAGATACCCTATTAT
Symbiotic markers	Wolbachia	16S rRNA	WspecF	YATACCTATTCGAAGGGATAG		Gel electrophoresis
Symbiotic markers	Wolbachia	16S rRNA	WspecR	AGCTTCGAGTGAAACCAATTC		Gel electrophoresis

ISSN: 1471-2180