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Fig. 4 | BMC Microbiology

Fig. 4

From: Nuclear and Wolbachia-based multimarker approach for the rapid and accurate identification of tsetse species

Fig. 4

Agarose gel electrophoresis (2% agarose) showing the Wolbachia amplicons for the different laboratory populations. The presence of the 438 bp amplicon is indicative of an active (cytoplasmic) Wolbachia infection, while the 296 bp amplicon is indicative of the presence of the partial sequence of the Wolbachia 16S rRNA gene that is integrated into the tsetse genome. Eight to twelve flies per laboratory population were analyzed. All abbreviations used in the Figures are shown in Additional file 5. The DNA ladder used to determine the size of the analyzed PCR products is also shown. #: Negative control during DNA extraction; +: positive PCR control (G. m. morsitans DNA).

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BMC Microbiology

ISSN: 1471-2180

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