Agarose gel electrophoresis (2.5% agarose) showing the ITS1 gene amplicons for the different tsetse laboratory populations. Eight to twelve flies per laboratory population were analyzed. All abbreviations used in the Figures are shown in Additional file 5. The DNA ladder used to determine the size of the analyzed PCR products is also shown. #: Negative control during DNA extraction; -: negative PCR control; +: positive PCR control (G. pallidipes DNA).