Fig. 4
From: High-sensitivity detection of cryptic Wolbachia in the African tsetse fly (Glossina spp.)
Stellaris® rRNA-FISH on testes of high-titer G. m. morsitans. a Wolbachia accumulating in the hub cells of the apical part (indicated by dotted box) of the testis. b In later stages of spermatogenesis, Wolbachia are present in 64 ‘onion stage’ spermatids. Wolbachia are stained in pink (16-23S rRNA), Glossina DNA is stained in blue (4′,6-diamidino-2-phenylindole). Minimum three individuals from each sex were dissected and processed for FISH. Abbreviations: GSC germline stem cells, SSC somatic stem cells, HC hub cells