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Fig. 3 | BMC Microbiology

Fig. 3

From: Analysis of the gut-specific microbiome from field-captured tsetse flies, and its potential relevance to host trypanosome vector competence

Fig. 3

Average taxonomic composition and % abundance of environmentally acquired and symbiotic bacteria found in midguts of uninfected and trypanosome infected G. pallidipes captured in Nguruman, Kenya, Shimba Hills, Kenya and Murchison Falls, Uganda. a Community structure of environmentally acquired bacteria (top set of graphs), and maternally transmitted symbionts (bottom set of graphs), identified from midguts of field-captured G. pallidipes. Data on the top and bottom graphs are presented as % abundance of the total (100%) bacterial population present in trypanosome uninfected and infected flies from each geographic location. The range of values on the Y-axes on the bottom set of graphs (maternally transmitted symbionts) is different so that low density Sodalis populations are visible. b Real-time quantitative PCR-based determination of Wigglesworthia and Sodalis densities in uninfected (UF) and trypanosome infected (IF) G. pallidipes trapped in Nguruman and Shimba Hills. Sample data derived from uninfected and parasite infected flies captured at each location was pooled so as to present an overall picture of the relationship between symbiont density and trypanosome infection status. Statistical significance of the data was determined by one-way ANOVA using GraphPad Prism software, v. 7

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