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Table 1 Bacterial strains used for the inclusivity and exclusivity tests in this study

From: An improved assay for rapid detection of viable Staphylococcus aureus cells by incorporating surfactant and PMA treatments in qPCR

Bacterial species

Strain

Source

Detection result by qPCR

Staphylococcus aureus (n = 20)

ATCC 25923

ATCCa

+

SA1011 #

Flour product

+

SA1012 #

Pork

+

SA1013 #

Frozen corn

+

SA1014 #

Raw milk

+

SA1015 #

Raw milk

+

SA1016 #

Raw milk

+

SA1017 #

Human (sputum)

+

SA1018 #

Human (blood)

+

SA1019 #

Human (pus)

+

SA1020 #

Human (wound)

+

SA1131 #

Human (sputum)

+

SA1132 #

Flour product

+

SA1133 #

Pork

+

SA1134 #

Frozen corn

+

SA1135 #

Raw milk

+

SA1136 #

Pig

+

SA1137 #

Pork

+

SA1138 #

Pig

+

SA1139 #

Raw milk

+

SA1140 #

Raw milk

+

Salmonella Typhimurium

ATCC 14028

ATCC

Salmonella Typhimurium

CICC21484

CICCb

Salmonella Enteritidis

CICC24119

CICC

Escherichia coli

ATCC 25922

ATCC

Listeria monocytogenes

CMCC54002

CMCCc

Listeria grayi

ATCC 25401

ATCC

Listeria seeligeri

ATCC 35967

ATCC

Listeria welshimeri

ATCC 35897

ATCC

Listeria ivanovii (n = 7)

ATCC 19119

ATCC

CMCC89001

CMCC

VP61 #

Human (anal swab)

VP65 #

Restaurant

VP67 #

Trout

VP105 #

Bass

VP106 #

Clams

Vibrio parahemolyticus

VP108 #

Cuttlefish

Streptococcus hemolyticus

ATCC 21059

ATCC

Campylobacter jejuni (n = 4)

ATCC 33291

ATCC

ATCC 43464

ATCC

CJ2 #

Human

CJ12 #

Chicken

Clostridium perfringens

ATCC 13124

ATCC

Bacillus cereus

ATCC 11778

ATCC

Pseudomonas aeruginosa

ATCC 27853

ATCC

  1. aATCC refers to American Type Culture Collection, USA
  2. bCVM-NAU refers to College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China
  3. cCMCC refers to National Center for Medical Culture, China