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Table 1 Primers used for detection of selected antibiotic resistance genes

From: Identification and antibiotic susceptibility of lactobacilli isolated from turkeys

Determining resistance to

Target gene

Primer sequence (5’→3’)

Amplicon size (bp)

Annealing temperature (°C)

Reference

tetracyclines

tetM

GTG GAC AAA GGT ACA ACG AG

CGG TAA AGT TCG TCA CAC AC

406

60

[20]

tetK

GAT CAA TTG TAG CTT TAG GTG AAG G

TTT TGT TGA TTT ACC AGG TAC CAT T

155

60

tetL

TGG TGG AAT GAT AGC CCA TT

CAG GAA TGA CAG CAC GCT AA

229

60

tetO

AAC TTA GGC ATT CTG GCT CAC

TCC CAC TGT TCC ATA TCG TCA

515

60

tetW

GAG AGC CTG CTA TAT GCC AGC

GGG CGT ATC CAC AAT GTT AAC

168

64

[50]

macrolides and lincosamides

ermA

CCC GAA AAA TAC GCA AAA TTT CAT

CCC TGT TTA CCC ATT TAT AAA CG

590

60

[20]

ermB

TGG TAT TCC AAA TGC GTA ATG

CTG TGG TAT GGC GGG TAA GT

745

60

mefA/E

CAA TAT GGG CAG GGC AAG

AAG CTG TTC CAA TGC TAC GC

317

60

ermC

AAT CGT CAA TTC CTG CAT GT

TAATCGTGGAATACGGGTTTG

299

58

[35]

lnuA

GGT GGC TGG GGG GTA GAT GTA TTA ACT GG

GCT TCT TTT GAA ATA CAT GGT ATT TTT CGA TC

323

61

[51]

aminoglycosides

aac(6’)-Ie-aph(2”)-Ia

CAG AGC CTT GGG AAG ATG AAG

CCT CGT GTA ATT CAT GTT CTG GC

348

56

[21]

aph3IIIa

GGC TAA AAT GAG AAT ATC ACC GG

CTT TAA AAA ATC ATA CAG CTC GCG

523

ant(4’)-Ia

CAA ACT GCT AAA TCG GTA GAA GCC

GGA AAG TTG ACC AGA CAT TAC GAA CT

294

aph(2”)-Ic

CCA CAA TGA TAA TGA CTC AGT TCC C

CCA CAG CTT CCG ATA GCA AGA G

444

aph(2”)-Id

GTG GTT TTT ACA GGA ATG CCA TC

CCC TCT TCA TAC CAA TCC ATA TAA CC

641

ant(6)-Ia

CGG GAG AAT GGG AGA CTT TG

CTG TGG CTC CAC AAT CTG AT

563

56

[52]

aac(6’)-Ii

TGGCCGGAAGAATATGGAGA

GCATTTGGTAAGACACCTACG

410

55

aadE

ATG GAA TTA TTC CCA CCT GA

TCA AAA CCC CTA TTA AAG CC

1060

51

[43]

chloramphenicol

cat

TAA GGT TAT TGG GAT AAG TTA

GCA TGR TAA CCA TCA CAW AC

340

54

[23]

tiamulin

lsaE

TGT CAA ATG GTG AGC AAA CG

TGT AAA ACG GCT TCC TGA TG

496

54

[53]

penicillins

blaZ

ACT TCA ACA CCT GCT GCT TTC

TAG GTT CAG ATT GGC CCT TAG

240

60

[54]

mecA

AGT TCT GCA GTA CCG GAT TTG C

AAA ATC GAT GGT AAA GGT TGG C

533

55

[55]

 

int-Tn (Tn916/Tn1545)

GCGTGATTGTATCTCACT GACGCTCCTGTTGCTTCT

1028

55

[49]