Fig. 5

Binding of CtrA to the flgB2-fliI2 regulatory region. DNA material immunoprecipitated with α-CtrA antibodies, was amplified using the flgBI2 and flgBI5 oligonucleotides. Samples were taken at different cycles and analyzed by agarose gel electrophoresis. Panel a and b show one representative experiment out of three independent assays. a) Samples of AM1 (WT) and EA1 (ΔctrA) carrying pRK_flgB2p::uidA-aadA (B2p-wt) were amplified during the number of cycles indicated below. The numbers in parenthesis represent the value obtained from the densitometric analysis. Input, shows the amplification products from an aliquot taken from each sample before ChIP (total chromatin input DNA). b) Samples of AR1 carrying pRK_flgB2p::uidA-aadA (WT) or the mutant versions pRK_flgB2p − 10::uidA-aadA (− 10) and pRK_flgB2p − 35::uidA-aadA (− 35), and EA1 carrying pRK_flgB2p::uidA-aadA (ΔctrA). The values in parenthesis represent the percentage of product relative to the amount detected for the wild type. Input shows the amplification products from an aliquot taken from each sample before ChIP