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Fig. 4 | BMC Microbiology

Fig. 4

From: Architecture of divergent flagellar promoters controlled by CtrA in Rhodobacter sphaeroides

Fig. 4

Architecture of the divergent promoters fliF2p-fliL2p and flgB2p-fliIp. The complete intercistronic region between these genes is shown. The coding region for FliF2, FliL2, FlgB2 and FliI2, represented by the initiation and 3 or 4 additional codons, is highlighted in green. The nucleotides matching the consensus CtrA-binding site are highlighted in yellow. For the intercistronic region between fliF2 and fliL2, the CtrA binding site is the same for both promoters; therefore, the highlighted nucleotides are on both strands. For the intercistronic region between flgB2 and fliI2, the CtrA binding site for fliI2p is highlighted on the top strand and the CtrA binding site for flgB2p is highlighted on the bottom strand. For reference the conserved A at the − 11 position is shaded in blue (on the top strand for fliI2p and on the bottom strand for flgB2p). The transcriptional start site is indicated by a bent arrow and shaded in pink. For the flgB2-fliI2 intercistronic region, a red bar below the sequence indicates the changes that the reduced the expression of flgB2p. A red bar above the sequence indicates the changes that reduced the expression of fliI2p. The orange bar below the sequence indicates the mutagenized position that did not affect the expression of flgB2p (− 23/− 24) but is located in the CtrA-binding site for fliI2p (highlighted in yellow in the top strand)

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