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Fig. 3 | BMC Microbiology

Fig. 3

From: Architecture of divergent flagellar promoters controlled by CtrA in Rhodobacter sphaeroides

Fig. 3

Activity of the wild-type and mutant versions of fliF2p, flgB2p, fliL2p and fliI2p in the AM1 strain. The amount of β-glucuronidase shown at the right, is expressed as percentage of the activity determined for the wild-type promoter. The values are the average of three independent experiments, SD was less than 25%. The nucleotides shaded in red represent the mutated bases regarding the wild-type promoter. The transcriptional start sites, are indicated by a bent arrow and the nucleotide is shaded in pink. The conserved A at − 11 is shaded in blue. The nucleotides matching with the consensus CtrA-binding site are highlighted in yellow. The translational codon is underlined. a) Activity of the wild-type and mutant promoters with changes affecting the A − 11 and the CtrA-binding site. b) Activity of the wild-type and mutant promoters affecting different regions in flgB2p and fliI2p. The underlined nucleotides upstream the transcriptional start site, indicate the mutagenized positions in the constructions tested in panel (a)

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