Table 1 Molecular identification of isolates based on partial 16S rRNA gene sequence information
From: Isolation and characterization of native probiotics for fish farming
Phylogenetic classification | Â | Consensus | BLAST analysis | No. of | New entry to | Pathogeni- | ||||
|---|---|---|---|---|---|---|---|---|---|---|
Phylum | Class | Family | Closest relative | Cluster | length [bp] | Accession no. | Similarity [%] | isolates | NCBI GenBank a | city |
Firmicutes | Bacilli | Bacillaceae | Bacillus cereus | Â | 860 | CP023245.1 | 96 | 1 | MH512875 | none |
|  |  | Staphylococcaceae | Staphylococcus saprophyticus |  | 887 | CP022093.1 | 100 | 1 | – | presumed |
|  |  |  | Staphylococcus sp. | 1 | 858 | MF417799.1 | 100 | 5 | – | none |
Actinobacteria | Actinobacteria | Dietziaceae | Dietzia sp. |  | 840 | KP713427.1 | 100 | 1 | – | none |
| Â | Â | Microbacteriaceae | Agrococcus sp. | Â | 830 | HM222687.1 | 99 | 1 | MH512857 | none |
|  |  |  | Microbacter. esteraromaticum |  | 859 | KJ812392.1 | 100 | 1 | – | none |
|  |  |  | Microbacter. phyllosphaerae |  | 857 | LT223598.1 | 100 | 3 | – | none |
|  |  |  | Microbacterium sp. | 1 | 830 | AM403655.1 | 100 | 3 | – | none |
| Â | Â | Micrococcaceae | Arthrobacter sp. | 1 | 842 | FR693359.1 | 97 | 1 | MH512858 | none |
| Â | Â | Â | Arthrobacter sp. | 2 | 839 | FR693359.1 | 99 | 2 | MH512865 | none |
|  |  |  | Kocuria palustris |  | 862 | MF033107.1 | 100 | 1 | – | none |
Proteobacteria | Alpha | Rhodobacteraceae | Paracoccus sp. |  | 808 | KJ576895.1 | 100 | 2 | – | none |
| Â | Beta | Comamonadaceae | Acidovorax sp. | 1 | 847 | KP967489.1 | 96 | 1 | MH512872 | none |
| Â | Â | Â | Acidovorax sp. | 2 | 839 | KP967489.1 | 98 | 1 | MH512871 | none |
| Â | Â | Â | Acidovorax sp. | 3 | 847 | KP967489.1 | 98 | 1 | MH512870 | none |
| Â | Â | Â | Acidovorax sp. | 4 | 839 | KP967489.1 | 99 | 1 | MH512874 | none |
| Â | Â | Â | Acidovorax sp. | 5 | 855 | KP967489.1 | 99 | 1 | MH512862 | none |
|  |  |  | Acidovorax sp. | 6 | 841 | KP967489.1 | 100 | 1 | – | none |
| Â | Â | Â | Acidovorax sp. | 7 | 838 | KP967499.1 | 98 | 1 | MH512863 | none |
| Â | Â | Â | Acidovorax sp. | 8 | 844 | KP967502.1 | 97 | 1 | MH512868 | none |
|  | Gamma | Aeromonadaceae | Aeromonas molluscorum |  | 852 | AY532692.1 | 100 | 2 | – | none |
| Â | Â | Â | Aeromonas media | Â | 852 | KP967511.1 | 99 | 1 | MH512885 | none |
|  |  |  | Aeromonas sp. | 1 | 858 | CP017143.1 | 100 | 3 | – | yes |
|  |  | Enterobacteriaceae | Buttiauxella sp. |  | 849 | LN824003.1 | 100 | 2 | – | none |
| Â | Â | Â | Serratia liquefaciens | Â | 862 | MF716555.1 | 99 | 1 | MH512879 | none |
|  |  | Oceanospirillaceae | Marinomonas sp. |  | 846 | JF710987.1 | 100 | 1 | – | none |
|  |  | Vibrionaceae | Aliivibrio finisterrensis |  | 868 | HM031457.1 | 100 | 2 | – | none |
| Â | Â | Â | Enterovibrio calviensis | Â | 852 | NR_041741.1 | 99 | 2 | MH512873 | none |
| Â | Â | Â | Enterovibrio nigricans | 1 | 852 | AM942724.1 | 99 | 14 | MH512886 | none |
| Â | Â | Â | Enterovibrio nigricans | 2 | 852 | AM942724.1 | 99 | 1 | MH512887 | none |
|  |  |  | Listonella anguillarum | 1 | 848 | CP011460.1 | 100 | 1 | – | yes |
| Â | Â | Â | Listonella anguillarum | 2 | 857 | CP023310.1 | 98 | 1 | MH512880 | yes |
| Â | Â | Â | Listonella anguillarum | 3 | 839 | CP023310.1 | 99 | 1 | MH512867 | yes |
|  |  |  | Listonella anguillarum | 4 | 864 | CP023310.1 | 100 | 16 | – | yes |
| Â | Â | Â | Photobacterium phosphoreum | 859 | AY888014.1 | 99 | 1 | MH512881 | yes | |
| Â | Â | Â | Photobacterium sp. | 1 | 837 | AB681340.1 | 99 | 1 | MH512869 | yes |
| Â | Â | Â | Photobacterium sp. | 2 | 859 | AB681340.1 | 99 | 1 | MH512882 | yes |
| Â | Â | Â | Photobacterium sp. | 3 | 855 | KC951114.1 | 96 | 1 | MH512883 | yes |
|  |  |  | Photobacterium sp. | 4 | 852 | KC951114.1 | 100 | 1 | – | yes |
|  |  |  | Vibrio aestuarianus |  | 864 | NR_113780.1 | 100 | 2 | – | none |
|  |  |  | Vibrio splendidus |  | 890 | JX441440.1 | 100 | 1 | – | yes |
| Â | Â | Â | Vibrio sp. | 1 | 866 | AB038029.1 | 99 | 1 | MH512877 | none |
| Â | Â | Â | Vibrio sp. | 2 | 839 | EU091323.1 | 99 | 1 | MH512866 | yes |
| Â | Â | Moraxellaceae | Acinetobacter haemolyticus | Â | 849 | KC178576.1 | 99 | 7 | MH512888 | none |
|  |  |  | Psychrobacter alimentarius |  | 833 | CP014945.1 | 100 | 13 | – | none |
|  |  |  | Psychrobacter cibarius |  | 868 | NR_043057.1 | 100 | 2 | – | none |
|  |  |  | Psychrobacter faecalis |  | 876 | JF710999.1 | 100 | 1 | – | none |
| Â | Â | Â | Psychrobacter sp. | 1 | 852 | FN377739.1 | 99 | 1 | MH512856 | none |
|  |  |  | Psychrobacter sp. | 2 | 846 | LC184492.1 | 100 | 3 | – | none |
| Â | Â | Â | Psychrobacter sp. | 3 | 870 | KT583340.1 | 99 | 3 | MH512861 | none |
| Â | Â | Â | Psychrobacter sp. | 4 | 876 | KT583340.1 | 99 | 2 | MH512864 | none |
| Â | Â | Â | Psychrobacter sp. | 5 | 852 | KU364058.1 | 98 | 1 | MH512860 | none |
| Â | Â | Â | Psychrobacter sp. | 6 | 852 | KU364058.1 | 99 | 1 | MH512859 | none |
|  |  |  | Psychrobacter sp. | 7 | 833 | KU364058.1 | 100 | 42 | – | none |
|  |  |  | Psychrobacter sp. | 8 | 876 | KX621125.1 | 100 | 1 | – | none |
| Â | Â | Shewanellaceae | Shewanella baltica | Â | 882 | AJ000216.1 | 99 | 5 | MH512878 | none |
| Â | Â | Â | Shewanella baltica | Â | 867 | CP000753.1 | 99 | 1 | MH512876 | none |
| Â | Â | Â | Shewanella baltica | Â | 862 | HM629402.1 | 99 | 2 | MH512884 | none |
|  |  |  | Shewanella baltica |  | 853 | HM629402.1 | 100 | 5 | – | none |
|  |  |  | Shewanella halifaxensis |  | 866 | NR_074822.1 | 100 | 1 | – | none |
|  |  |  | Shewanella sp. | 1 | 866 | FR744880.1 | 100 | 3 | – | none |
|  |  |  | Shewanella sp. | 2 | 868 | JQ765408.1 | 100 | 3 | – | none |
|  |  |  | Shewanella sp. | 3 | 853 | LK392315.1 | 100 | 10 | – | none |
|  |  |  |  |  |  |  | ∑ | 195 |  |  |