Fig. 6From: Pseudomonas aeruginosa responds to exogenous polyunsaturated fatty acids (PUFAs) by modifying phospholipid composition, membrane permeability, and phenotypes associated with virulenceIncubation with exogenous fatty acids alters biofilm formation in Pseudomonas aeruginosa. Overnight cultures were pelleted, washed, resuspended in appropriate media and inoculated onto microtiter plates (starting OD ~ 0.1) in octuplet. Each culture was grown in the presence of 300 μM of the indicated fatty acids. After 24 h incubation, the biofilm assay by O’Toole was performed in media buffered at two pH levels (5.5 and 7.4). The absorbance (OD590) was measured using a Biotek Synergy microplate reader. Each value represents the mean and standard deviation of 8 wells. Asterisks indicate p-values determined to be less than 0.008 when compared to the no fatty acid controlsBack to article page