Fig. 3
The effect of exogenous fatty acids on hydrophobic compound uptake in Pseudomonas aeruginosa. a Bacteria were grown at 37 °C in G56 (pH 7.4) with and without 300 μM of the indicated fatty acids to mid-log phase (OD = 0.8). Cultures were gently pelleted, washed with PBS and resuspended in an equal volume of PBS (OD600 = 0.4). The amount of CV in the supernatant following centrifugation was measured at regular intervals and expressed graphically as percentage of CV uptake. All standard deviations were less than 3% (not graphed for visual clarity). b Bacteria were grown at 37 °C in CM9 with and without 300 μM of the indicated fatty acids to mid-log phase (OD = 0.8). Cultures were gently pelleted, washed with PBS and resuspended in an equal volume of PBS (OD600 = 0.7). The amount of EtBr in the supernatant following centrifugation was measured as fluorescence emission intensity at 585 nm (excitation wavelength of 530 nm). Asterisks indicate significant difference (*, p < 0.001) as compared to control. c Bacteria were grown at 37 °C in CM9 with and without 300 μM of the indicated fatty acids to mid-log phase (OD = 0.8). Cultures were gently pelleted, washed with PBS and resuspended in an equal volume of PBS (OD600 = 0.4). Following addition of 20 μM EtBr, fluorescence intensity was measured every 5 m for 1 h (excitation wavelength of 550; emission wavelength of 600)