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Fig. 2 | BMC Microbiology

Fig. 2

From: Pseudomonas aeruginosa responds to exogenous polyunsaturated fatty acids (PUFAs) by modifying phospholipid composition, membrane permeability, and phenotypes associated with virulence

Fig. 2

Ultra performance liquid chromatography/mass spectrometry of isolated phospholipids from Pseudomonas aeruginosa grown in the presence and absence of fatty acids. P. aeruginosa was grown with or without 300 μM of a given fatty acid at 30 °C in G56 (pH 7.4) to logarithmic phase. Lipids were extracted using the Bligh and Dyer method but included an extra wash step to increase the purity of the isolated lipids. The lipid extract was injected (5 μL) into a Waters UPLC for gradient elution using a reversed phase C-8 column. [M-H] ions were detected by quadrupole mass spectrometry following electrospray ionization. a Total ion chromatograms (m/z 650–850) comparing the control to cultures exposed to either 18:3α or 22:6 fatty acid. Changes in the chromatograms compared to the control suggest modifications of the phospholipid profile that depend on the supplied fatty acid. b Extracted ion chromatograms (XIC) showing new peaks, in the exposed samples, that are absent in the control. The labeled peaks are predicted using the Lipid Maps Database ( http://www.lipidmaps.org/ ) and are based on the m/z of the parent ion. From left to right, the XIC’s correspond to the following m/z values: 743.5, 769.5, 793.5, 712.5, 738.5, 762.5, 788.5. The control was mass filtered for all of these same ions but only noise was present. As a result, only the XIC at 793.5 is used to represent the control

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