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Table 1 Bacterial strains and plasmids used in this study

From: The alternative sigma factor RpoQ regulates colony morphology, biofilm formation and motility in the fish pathogen Aliivibrio salmonicida

Bacterial strains or plasmids Description Source
A. salmonicida
 LFI1238 Wild-type, isolated from Atlantic cod [18]
 ΔlitR LFI1238 containing an in-frame deletion in litR [22]
 ΔrpoQ LFI1238 containing an in-frame deletion in rpoQ This study
 ΔrpoQc ΔrpoQ strain complemented with wild-type copy of the rpoQ gene, Cmr This study
 ΔlitR-rpoQ ΔlitR stain with an insertional disruption in rpoQ, Cmr This study
 LFI1238-pVSV102 LFI1238 carrying pVSV102, Knr This study
 ΔlitR-pVSV102 ΔlitR carrying pVSV102, Knr This study
 ΔrpoQ-pVSV102 ΔrpoQ carrying pVSV102, Knr This study
 LFI1238-pTM214 LFI1238 carrying pTM214, Cmr This study
 LFI1238-Ptrc-rpoQ LFI1238 carrying pTM214-rpoQ, Cmr This study
 ΔlitR-pTM214 ΔlitR carrying pTM214, Cmr This study
 ΔlitR-Ptrc-rpoQ ΔlitR carrying pTM214-rpoQ, Cmr This study
E. coli
 S17λpir Donor strain for conjugation [65]
 JM109 Strain for subcloning pGEM-T constructs [66]
 DH5α Strain for cloning Thermo Fisher
 C118λpir Helper strain containing pEVS104 [32]
 DH5αλpir Donor strain for conjugation harboring pVSV102 [32]
 PIR2 Donor strain for conjugation harboring pTM214 [33]
Plasmids
 pDM4 Suicide vector with an R6K origin, sacBR and Cmr [35]
 pNQ705 Suicide vector with an R6K origin, Cmr [35]
 pDM4-ΔrpoQ pDM4 containing a fragment of rpoQ harboring an internal deletion This study
 pNQ705-rpoQc pNQ705 containing a full length rpoQ and flanking sequences This study
 pNQ705-rpoQ pNQ705 containing an internal 304 bp fragment of rpoQ This study
 pTM214 pVSV105, Ptrc-mCherry, Cmr [33]
 pVSV102 pES213, constitutive GFP, Knr [67]
 pEVS104 Helper plasmid, R6K origin, RP4, oriT, trb, tra and Knr [32]
 pTM214-rpoQ pVSV105, Ptrc-rpoQ (a full length rpoQ copy), Cmr This study
 pGEM-T TA cloning vector, white/blue screening, Ampr Promega