|
A. salmonicida
|
|
LFI1238
|
Wild-type, isolated from Atlantic cod
|
[18]
|
|
ΔlitR
|
LFI1238 containing an in-frame deletion in litR
|
[22]
|
|
ΔrpoQ
|
LFI1238 containing an in-frame deletion in rpoQ
|
This study
|
|
ΔrpoQc
|
ΔrpoQ strain complemented with wild-type copy of the rpoQ gene, Cmr
|
This study
|
|
ΔlitR-rpoQ−
|
ΔlitR stain with an insertional disruption in rpoQ, Cmr
|
This study
|
|
LFI1238-pVSV102
|
LFI1238 carrying pVSV102, Knr
|
This study
|
|
ΔlitR-pVSV102
|
ΔlitR carrying pVSV102, Knr
|
This study
|
|
ΔrpoQ-pVSV102
|
ΔrpoQ carrying pVSV102, Knr
|
This study
|
|
LFI1238-pTM214
|
LFI1238 carrying pTM214, Cmr
|
This study
|
|
LFI1238-Ptrc-rpoQ
|
LFI1238 carrying pTM214-rpoQ, Cmr
|
This study
|
|
ΔlitR-pTM214
|
ΔlitR carrying pTM214, Cmr
|
This study
|
|
ΔlitR-Ptrc-rpoQ
|
ΔlitR carrying pTM214-rpoQ, Cmr
|
This study
|
|
E. coli
|
|
S17λpir
|
Donor strain for conjugation
|
[65]
|
|
JM109
|
Strain for subcloning pGEM-T constructs
|
[66]
|
|
DH5α
|
Strain for cloning
|
Thermo Fisher
|
|
C118λpir
|
Helper strain containing pEVS104
|
[32]
|
|
DH5αλpir
|
Donor strain for conjugation harboring pVSV102
|
[32]
|
|
PIR2
|
Donor strain for conjugation harboring pTM214
|
[33]
|
|
Plasmids
|
|
pDM4
|
Suicide vector with an R6K origin, sacBR and Cmr
|
[35]
|
|
pNQ705
|
Suicide vector with an R6K origin, Cmr
|
[35]
|
|
pDM4-ΔrpoQ
|
pDM4 containing a fragment of rpoQ harboring an internal deletion
|
This study
|
|
pNQ705-rpoQc
|
pNQ705 containing a full length rpoQ and flanking sequences
|
This study
|
|
pNQ705-rpoQ−
|
pNQ705 containing an internal 304 bp fragment of rpoQ
|
This study
|
|
pTM214
|
pVSV105, Ptrc-mCherry, Cmr
|
[33]
|
|
pVSV102
|
pES213, constitutive GFP, Knr
|
[67]
|
|
pEVS104
|
Helper plasmid, R6K origin, RP4, oriT, trb, tra and Knr
|
[32]
|
|
pTM214-rpoQ
|
pVSV105, Ptrc-rpoQ (a full length rpoQ copy), Cmr
|
This study
|
|
pGEM-T
|
TA cloning vector, white/blue screening, Ampr
|
Promega
|
