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Fig. 7 | BMC Microbiology

Fig. 7

From: Hpa1 is a type III translocator in Xanthomonas oryzae pv. oryzae

Fig. 7

Hpa1 α-helices play a critical role in PthXo1 translocation. a–e, Bacterial hpa1 mutants ∆N36, ∆Nα, ∆Cα, and αNC were generated by deleting hpa1 sequence regions, which encode the N-terminal region made of 36 residues, the N-terminal α-helix, the C-terminal α-helix, and both α-helices, respectively. Resulting mutant genes were introduced into the ∆hpa1 or ∆hpa1∆pthXo1/pthXo1-cya mutant of Xoo strain PXO99. Recombinant bacteria were used in Nipponbare inoculation by the leaf-top-clipping method. Inoculated plants were subjected to the following analyses. (a) Bacterial blight symptoms on leaves photographed at day 12 after inoculations. b Blight lesion length on leaves from (a). c Relative units (r.u.) of OsSWEET11 gene expression in leaves at 12 hpi. The average expression level of OsSWEET11 in the plant with Δhpa1/hpa1ΔNC α-helix was defined as 1 to evaluate relative extents of gene expression in plants inoculated with other Xoo strains. (d) The content of cAMP from PthXo1-Cya activity in cytoplasm of leaf cells at 12 hpSi. In (b) to (e), data are the means ± SEMs; different letters on bar graphs indicate significant differences in multiple comparisons for the bacterial strains; P < 0.05; n = 9 repetitions from 3 independent experiments each involving 3 repetitions

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