Fig. 5

Hpa1 is a type III translocator determined to be required for translocation of PthXo1 and AvrXa21 as well as for their target gene expression. (a) and (b) Bacterial effector translocation levels shown as cAMP concentrations in leaves of Nipponbare or IRBB10 plants 12 hpi with Xoo strains, which are related to hpa1, pthXo1, or the secretion determinant gene hrcV. (c) and (d) Bacterial populations in leaves of plants 12 hpi with the indicated bacterial strains. Quantitative data are given as the means ± SEMs. (e) Rice OsSWEET11 gene expression in leaves of plants equivalent to those in (a). The average level of gene expression in plants inoculated with the hrcV mutant was defined as 1 to assess relative extents of gene expression in other plants. (f) Rice Xa10 gene expression in leaves of plants equivalent to those in (b). The average level of gene expression in plants inoculated with the hrcV mutant was defined as 1 to assess relative extents of gene expression in other plants. On bar graphs in (a) to (f), different letters indicate significant differences of multiple comparisons of data from the different bacterial strains; P < 0.05; number of repetition (n) = 9 from 3 independent experiments each involving 3 repetitions