| NanoDrop measurements (mean ± SD) |
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DNA concentration, ng/μL | 260 nm/280 nm ratio | 260 nm/230 nm ratio |
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Fresh + QIA | 304.60 ± 106.35 | 1.86 ± 0.02 | 1.67 ± 0.20 |
O24h + QIA | 267.22 ± 98.38a | 1.88 ± 0.01 | 1.66 ± 0.16b |
O7d + QIA | 223.27 ± 100.03 | 1.88 ± 0.02 | 1.60 ± 0.19 |
O24h + PF | 41.31 ± 32.52a | 2.09 ± 0.39 | 1.02 ± 0.50b |
- The ratios of absorbance at 260 nm/280 nm and 260 nm/230 nm are used to assess the purity of DNA. A ratio of ~ 1.8 is generally accepted as “pure” for DNA. If the ratio is appreciable lower, it may indicate the presence of contaminants (e.g. proteins, phenols or carbohydrates) (13, 14). aIs the comparison of DNA concentration (column labeled DNA concentration, ng/μL) and bof DNA purity (column labeled 260 nm/230 nm ratio) between QIA and PF DNA extraction methods (Bonferroni-adjusted paired sample t-test with P < 0.001 for all tests)