Reliability of a participant-friendly fecal collection method for microbiome analyses: a step towards large sample size investigation

Table 1 Primers and probes used to carry out real time PCR quantification of Bacteroides spp., Bifidobacterium spp. and Clostridium cluster IV

Target	Name	Sequence	Amplicon Length	Concentration nM	Ref.
Clostridium cluster IV	sg-Clep-F	GCA CAA GCA GTG GAG T	239	400	[29]
	sg-Clep-R	CTT CCT CCG TTT TGT CAA		400
	Clep-Pa	(FAM)-AGG GTT GCG CTC GTT-(TAMRA)		200
Bifidobacterium spp.	F-bifido	CGCGTCYGGTGTGAAAG	244	300	[30]
	R-bifido	CCCCACATCCAGCATCCA		300
	MGB-bifido	(FAM)-AACAGGATTAGATACCC-(MGB)		200
Bacteroides spp.	AllBac296f	GAG AGG AAG GTC CCC CAC	106	400	[31]
	AllBac412r	CGC TAC TTG GCT GGT TCA G		400
	AllBac375Bhqr	(FAM)-CCA TTG ACC AAT ATT CCT CAC TGC TGC CT-(TAMRA)		200

Target Selected species (spp.) tested with qPCR, Name Name of primers and probes used to amplify Target, Sequence primers/probes sequence, Amplicon length the PCR product size of the Target in base pars (bp), Concentration nM concentration of the primers/probes needed to perform experiment, nM nanomolar, Ref. references where the method is described

ISSN: 1471-2180