Table 2 Primers and temperature conditions used for PCR [29]
Locus | Primer | Annealing temp (°C) | Cycles | Direction | Sequence (5′-3′) | Reference |
|---|---|---|---|---|---|---|
CaM | CMD5 | 55 | 35 | Forward | CCGAGTACAAGGARGCCTTC | [53] |
CMD6 | 55 | 35 | Reverse | CCGATRGAGGTCATRACG TGG | ||
BenA | Bt2a | 55 | 35 | Forward | GGTAACCAAATCGGTGCTGTTC | [54] |
Bt2b | 55 | 35 | Reverse | ACCCTCAGTGTAGTGACCCTTGGC | ||
MAT1–1 | AFM1_F65655 MAT1_R6215 | 48 48 | 30 30 | Forward Reverse | CCTYGACGMGATGGGITGG TGTCAAAGARTCCAAAAGGAGG | this study |
MAT1–2 | MAT2_F6086 MAT2_R6580 | 48 48 | 30 30 | Forward Reverse | TCGACAAGATCAAAWCYCGTC CTTYTTGARCTCTTCYGCTAGG | this study |