Fig. 6
The activity of (a) V.NgoAXIII endonuclease and (b) V.NgoAXIV endonuclease in the presence of MutLE.coli protein encoded by E. coli. Histograms presenting first-order rate constants (kst) obtained for endonucleolytic reactions catalyzed by gonococcal Vsr endonucleases in the presence and absence of MutLE.coli protein. Reactions were carried out using 0.15 μM substrate DNA, 0.3 μM or 1.5 μM Vsr endonuclease and, where indicated, 0.6 μM MutLE.coli protein, and first-order rate constants (kst) were determined. Asterisks indicate statistically significant differences, which were calculated using the two-tailed heteroscedastic Student’s t-test (p value < 0.05)