Fig. 2

The interactions between Vsr endonucleases, MutL and MutS proteins of N. gonorrhoeae FA1090. The study was carried out using the bacterial two-hybrid system with lacZ as a reporter gene as described in the Methods section. The β-galactosidase activity was estimated and presented as Miller Units, and the ratio between E. coli cells harboring plasmids with genes encoding proteins under investigation to plasmid-less E. coli was calculated. Black bars indicate the presence of interactions between the investigated proteins. The results are the mean values obtained from at least six independent repeats. Asterisks indicate statistically significant differences, calculated using the two-tailed heteroscedastic Student’s t-test (p value < 0.05)