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Table 2 The reaction system components and procedures of Q-PCR

From: Differences in the digestive enzyme activity, intestinal mucosa and microbial community in loach cultivated in two separate environments

components SYBR® Premix Ex Taq™ II forward and reverse primer (10 μM) template DNA sterile deionized water total
volume 12.5 μl 2 μl 1 μl 9.5 μl 25 μl
reaction procedures predenaturation 40 cycles extension
   denaturation annealing extension  
Temperature (°C) 95 94 optimal temperature 72 72
Time (s) 60 15 30 30 10