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Table 2 The reaction system components and procedures of Q-PCR

From: Differences in the digestive enzyme activity, intestinal mucosa and microbial community in loach cultivated in two separate environments

components

SYBR® Premix Ex Taq™ II

forward and reverse primer (10 μM)

template DNA

sterile deionized water

total

volume

12.5 μl

2 μl

1 μl

9.5 μl

25 μl

reaction procedures

predenaturation

40 cycles

extension

  

denaturation

annealing

extension

 

Temperature (°C)

95

94

optimal temperature

72

72

Time (s)

60

15

30

30

10