Fig. 2

Field inversion gel electrophoresis (FIGE) analysis of phage DNA. (A1) FIGE analysis of EcoRI- digested phage DNA induced from toxin-producing E. coli O157 isolates and (A2) its corresponding blot hybridized with radioactively labeled stx₂ probe. Lane 1: M18 (undigested), Lane 2: M18 (digested), Lane 3: M133 (undigested), Lane 4: M133 (digested), Lane 5: 1 kb Marker, Lane 6: M163 (undigested), Lane 7: M163 (digested), Lane 8: G51 (undigested), Lane 9: G51 (digested), Lane 10: G71 (undigested), Lane 11: G71 (digested). (B1) FIGE analysis of phage DNA induced and uninduced from toxin-producing E. coli O157 isolates and (B2) its corresponding blot hybridized with radioactively labeled stx₂ probe. Lane 1: M18 (uninduced), Lane 2: M18 (induced), Lane 3: M133 (uninduced), Lane 4: M133 (induced), Lane 5: G71 (uninduced), Lane 6: G71 (induced), Lane 7: M163 (uninduced), Lane 8: M163 (induced), Lane 9: G51 (uninduced), Lane 10: G51 (induced). (C1) FIGE analysis of phage DNA induced and uninduced from E. coli O157 isolates producing little or no toxin and (C2) its corresponding blot hybridized with radioactively labeled stx₂ probe. Lane 1: C96 (uninduced), Lane 2: C96 (induced), Lane 3: M143 (uninduced), Lane 4: M143 (induced), Lane 5: M168 (uninduced), Lane 6: M168 (induced), Lane 7: M171 (uninduced), Lane 8: M171 (induced), Lane 9: M173 (uninduced), Lane 10: M173 (induced)