Table 2 Primers used in this work
Primer name | Primer sequence | Product length (bp) |
|---|---|---|
For construction | ||
 sacB-F | CCCTCTAGA ATCAAAAAGTTTGCAAAACAAG |  |
 sacB-R | CCCGTCGAC AAATAAAAGAAAATGCCAATAG | 1419 |
 RpfC-1-FOR | ATTGCGCTGATCCTGGTCTACAC |  |
 RpfC-1-REV | CGGGATCC AGACTTCATAGACGCCTCAGACG | 553 |
 RpfC-2-FOR | CGGGATCC CGTAGCAACGAATAGACCGC |  |
 RpfC-2-REV | ACAGCGACGTGTTCAATCTGGGCG | 665 |
 PhrpG-F | GGGGAGCTC GGTGTTCGGCACGCAGATGCGC |  |
 PhrpG-R | GGGTCTAGA GTCCATCACTCGCGCGCCCACG | 590 |
 PhrpX-F | GGGGAATTC CTGACGCATAGGGCTGGTTGGGGC |  |
 PhrpX-R | GGGGGTACC CTGGAGGTGCTGCAGACCCTGTGG | 677 |
For sequencing | ||
 KAN-2 FP-1 | ACCTACAACAAAGCTCTCATCAACC |  |
 KAN-2 RP-1 | GCAATGTAACATCAGAGATTTTGAG |  |
For qReal-time PCR | ||
 XC0052F | ACAGATTGGTCTCGCAGGTC | 104 |
 XC0052R | GGCAATGCTCTGATCGGTCT |  |
 XC0241F | AGCCGCATCCACGAAACGGA | 92 |
 XC0241R | AACAGCGCGGTGCGTCGTAA |  |
 XC1553F | TTTTCCGGATGGCTCGAACA | 108 |
 XC1553R | AGGATGCAGACTGACCAAGC |  |
 XC2004F | TTGAGGCGGCCATATCACTC | 119 |
 XC2004R | CCACACTGCCGATACACCTT |  |
 XC2081F | AGGAAGTGCGGATGAACCTG | 141 |
 XC2081R | CGCCGAAACCATTTCGAGAC |  |
 XC2602F | TCGAGGATCCGCAAACTACG | 110 |
 XC2602R | GACCGGCATCGAGGAAAAGA |  |
 XC2994F | CTCCTGCCATCTTGAGCGAT | 122 |
 XC2994R | CGCAATCAGCATGAAGTCCG |  |
 XC2995F | CACGTGGGGCGAGAAAGATA | 116 |
 XC2995R | GCCGTTGGAACAAGGGAGTA |  |
 XC3160F | GCTCGCAAGTCTGATGGAGT | 126 |
 XC3160R | CATGACGACAGACCCAGCTT |  |
 XC3177F | ATGGACTCAGCGTTGTGGAG | 110 |
 XC3177R | TCATTGTTTCGTGGCAAGCG |  |
 XC3802F | TTTCGACGATCTTCCCGAGC | 111 |
 XC3802R | TGGATGGAGGTGTTGTACGC |  |
 XC4273F | CGGCGCGGAGTTAAATCTTG | 129 |
 XC4273R | AAAGTCTGCTCCGGGAATCG |  |
 XC3076F | CGAAGTCGCATTGCTGGGCG | 93 |
 XC3076R | GCCTTGGACGCCTGCCGATA |  |
 XC3077F | TGCGTGGCATCGGACGACAG | 92 |
 XC3077R | CACTCGAAACGGCCCAGCAC |  |
 XC3002F | CCTGCAGACGATGGGCATCG | 188 |
 XC3002R | CGTCCTGTTGACCGCTCTGC |  |
 XC3003F | CGTTACCTGATGACGCGCGT | 155 |
 XC3003R | AGGTCGGCGGATGCATAACC |  |
 XC3004F | GCCTGGTGGGGCTGGTGTTCAA | 164 |
 XC3004R | CGTGCTCTGCTCACCGCTCA |  |
 XC3005F | TGCAGCAGCTGAAGACGCGC | 200 |
 XC3005R | CAGGATCGCCTCGATGCCGA |  |
 XC3006F | CGCCGTTTGGCGAGCTGGTGGG | 179 |
 XC3006R | CGCCTGCGCCTGGATCTGCA |  |
 XC3007F | GCAGGCGCTGGCGGACGTCC | 169 |
 XC3007R | CACGCCGCGCTCGTTCCACG |  |
 XC3008F | CCGTGTCCACGCTGGCGCAA | 150 |
 XC3008R | CGCCGACCTGCATGCTCGCC |  |
 XC3009F | ACGGCCGGTGTGGATGCAGA | 177 |
 XC3009R | GGGTGTGGAGATCAGGCCGT |  |
 XC3010F | GCTGATGCAATCCTCCTGCC | 151 |
 XC3010R | CCCCATCTTTGGCGCATTGG |  |
 XC3011F | GCGAGTACTGCGGCCAGAGT | 153 |
 XC3011R | CAACACGCGTACAAGGCCTT |  |
 XC3012F | TTGTGCAGACCGGGCTTAAT | 160 |
 XC3012R | TACCACAGCACCACGCCGAT |  |
 XC3014F | GGATTGCCGGACACGGTGGT | 150 |
 XC3014R | TCGGGCGATCTGTCGACGAT |  |
 XC3015F | TGGAACCACTGGGACTAGGCG | 159 |
 XC3015R | CAGCGCTAGCCGTTTGCAGC |  |
 XC3016F | AATGCCATCGGCGTGCAGCA | 172 |
 XC3016R | CGCGACAGGCATCGAGCAAT |  |
 XC3017F | GTGCGATTCACTTCCGAAGC | 155 |
 XC3017R | ACCACCACCAGCTTGAGCGC |  |
 XC3018F | GAACTGGAAGAAGCCGAAGCG | 192 |
 XC3018R | ACGGGCGCTGTCGTCTACCT |  |
 XC3019F | AGATTGGCCTGATTGTTCGC | 178 |
 XC3019R | CTCCAGCAGCGCAACATCGT |  |
 XC3020F | CACGCTCACCCAGGATATGA | 163 |
 XC3020R | GACAATGAAATCGTTGCGCG |  |
 XC3021F | GATTGGGCCAGGCCAGGGAT | 168 |
 XC3021R | CGTTCTTCTTCGCGGTCAGG |  |
 XC3022F | CACATGCCTGCAGCCCAGAC | 154 |
 XC3022R | CCTGTGCGTACACCGACAAA |  |
 XC3023F | CGCGCCACCCGGCCTCCAGA | 185 |
 XC3023R | CGCCGCCGCCCTTCATGTTG |  |
 XC3024F | GTGCTGGGCCGTCACATGCT | 155 |
 XC3024R | ACCGCCTGCTGCACGACCGT |  |
 XC3025F | GTTGCCGCCTGCGGTGGATG | 188 |
 XC3025R | GCAAGCCTTGCAGCGCACTC |  |
 XC1331F | TGTGCCTGGATTCGGGTTGC | 323 |
 XC1331R | CCACCATCGGAAACTTGTCG |  |
 XC3907F | CGATGTTCGCCACCCACAAC | 318 |
 XC3907R | GGATGGACGCAAACGAGGAC |  |
 XC3379F | CAACGATGCGTCCAATGTGTC | 301 |
 XC3379R | CAAGGTTTCCACCGCTGCTG |  |
 XC1969F | CGGCTACAAGAACGCCTACCCG | 156 |
 XC1969R | GCGATGTCCTGCTCGGAAAAGC |  |
 XC2272F | GAGCCCTGAAATCGCCCTGACC | 223 |
 XC2272R | CTCCACCAGATGTCCCAGCAGC |  |
 XC3324F | GTCTTCACTGCCGACGGTTC | 164 |
 XC3324R | TCGAATGCGACCTTCTCGATAC |  |
 XC4122F | TTCGTATGATTTCCTCGGCC | 142 |
 XC4122R | TACTTGATCTTGCCTTCCTTGT |  |
 XC1019F | ACACGATTTCTGGGTTTTGCGC | 304 |
 XC1019R | ATTCAGTGCGTTGAGTTCTGGC |  |
 XC3862F | AGGCAAGCCCCGAATCCGAAGC | 251 |
 XC3862R | CACGGCGTCGTCCAGTGTGTTG |  |
 XC4147F | ACGGCTACATCGGGTTGATC | 197 |
 XC4147R | TCATTTGCGGGCTTCCTCC |  |
 XC2979F | ATGAGCGACTGGGAAGGACG | 231 |
 XC2979R | GGCAAACTGCTTGAGGTCAG |  |
 XC0109F | GCGAAAAACGCCTGGCGGTGC | 166 |
 XC0109R | AGCTTGCCGGCATCCAGCGC |  |
 XC0705F | CTACTGGCGTGACGTTGGTG | 156 |
 XC0705R | CACCCATCACACCGGACCTG |  |
 XC1002F | CACTGCGTTATGTGCTGCCC | 158 |
 XC1002R | CAGTTTCGACGCGGCAATGG |  |
 XC1850F | GGCAGCACGCGCCGCTACATCAG | 151 |
 XC1850R | TGGGCGTGGGGTTGGCATTG |  |
 XC2254F | GAACTGGAACGTTGCCTGGG | 150 |
 XC2254R | GTGCGATGTCGCGACGAAGC |  |
 XC1621F | GATCTGTGGAAGCAGTAACG | 159 |
 XC1621R | CTACTCGGGCCTTGAACAAC |  |
 XC2512F | CGCGTGCGCGTAACGGTGTG | 150 |
 XC2512R | CGCTACGCGTGAAGCTGGGG |  |
 XC0155F | GCGTGTTGCGCAGCTTCGAAC | 168 |
 XC0155R | GCATGCGCATCAGCTTGAGG |  |
 XC1978F | CTCAAGCTGCGCGGCCATCC | 151 |
 XC1978R | GCACCATTGCGCGCCCCAGC |  |
 XC1294F | GCGCGCAGCCAGTGCCGTGG | 131 |
 XC1294R | CGGTGCCGGCGACTGCCACT |  |
 XC2088F | GCGAGTGGAAAAACCAGCTGGGT | 140 |
 XC2088R | AACCGGGTTGGCAAACCAGC |  |
 XC3540F | TGAGCGTGCCAACAAGGACT | 152 |
 XC3540R | ATTCGACCTTGGTGCGCAGC |  |
 XC3697F | GGCGACAGGCCCGCGGATGGTTGT | 144 |
 XC3697R | GCCCGCAGGCCCAGCCGAAT |  |
 16S-F | GAGGAAGGTGGGGATGACGTCA | 108 |
 16S-R | GATTGGCTTACCCTCGCGGG |  |