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Table 1 Bacterial strains and plasmids used in this work

From: RpfC regulates the expression of the key regulator hrpX of the hrp/T3SS system in Xanthomonas campestris pv. campestris

Strains or plasmids Relevant characteristicsa Source
X. c. pv. campestris
 8004 Wild type; Rifr [24]
 XB001 8004/pL6hrpXsacB with a Tn5 insertion in XC_4007; Rifr; Kanr; Tcr This work
 XB002 8004/pL6hrpXsacB with a Tn5 insertion in the intergenetic region This work
  between the ORFs XC_1510 and XC_1511; Rifr; Kanr; Tcr  
 XB003 8004/pL6hrpXsacB with a Tn5 insertion in XC_2333; Rifr; Kanr; Tcr This work
 XB004 8004/pL6hrpXsacB with a Tn5 insertion in XC_1192; Rifr; Kanr; Tcr This work
 XB005 8004/pL6hrpXsacB with a Tn5 insertion in XC_3951; Rifr; Kanr; Tcr This work
 XB006 8004/pL6hrpXsacB with a Tn5 insertion in XC_0124; Rifr; Kanr; Tcr This work
 8004/pL6hrpXsacB 8004 harboring plasmid pL6hrpXsacB; Rifr; Tcr This work
ΔrpfC rpfC in frame deletion mutant of 8004; Rifr This work
CΔrpfC ΔrpfC harboring plasmid pLCrpfC; Rifr; Tcr This work
ΔrpfG rpfG in frame deletion mutant of 8004; Rifr [17]
ΔavrBs1 avrBs1 in frame deletion mutant of 8004; Rifr; Gmr [44]
 8004/pGUShrpG 8004 harboring plasmid pGUShrpG; Rifr; Tcr This work
ΔrpfC/pGUShrpG ΔrpfC harboring plasmid pGUShrpG; Rifr; Tcr This work
 8004/pGUShrpX 8004 harboring plasmid pGUShrpX; Rifr; Tcr This work
ΔrpfC/pGUShrpX ΔrpfC harboring plasmid pGUShrpX; Rifr; Tcr This work
E. coli
 JM109 RecA1, endA1, gyrA96, thi, supE44, relA1 Δ(lac-proAB)/F′ [traD36, lacIq, lacZ ΔM15] [29]
Plasmids
 pUC19 Cloning vector; Ampr [28]
 pLAFR6 Broad host range IncP cloning cosmid; Tcr [28]
 pK18mobsacB Suicide plasmid in Xcc; Mob+ Tra; Kanr [27]
 pLGUS pLAFR6 containing a 1832-bp gusA ORF (excluding ATG), Tcr [31]
 pL6sacB pLAFR6 containing a 1419-bp sacB gene, Tcr This work
 pKrpfCsacB pK18mobsacB containing the two flanking fragments of rpfC; Kanr This work
 pUCPhrpG pUC19 containing hrpG promoter; Ampr This work
 pUCPhrpX pUC19 containing hrpX promoter; Ampr This work
 pGUShrpG pLAFR6 containing hrpG promoter in frame fused with gus gene; Tcr This work
 pGUShrpX pLAFR6 containing hrpX promoter in frame fused with gus gene; Tcr This work
 pL6hrpXsacB pLAFR6 containing hrpX promoter in frame fused with sacB gene; Tcr This work
 pLCrpfC pLAFR6 containing the sequenced whole ORF of rpfC; Tcr This work
  1. aAmpr, ampicillin-resistant; Gmr, gentamicin-resistant; Kanr, kanamycin-resistant; Rifr, rifampicin-resistant; Tcr, tetracycline-resistant