Fig. 3
RpfC positively affects the expression of hrpG and hrpX in host plant. Xcc strains 8004/pGUShrpG, 8004/pGUShrpX, ΔrpfC/pGUShrpG, and ΔrpfC/pGUShrpX were cultured in NYG medium overnight and resuspended in water to an optical density at 600 nm of 0.01, and then inoculated into the Chinese radish cv. Manshenhong leaves by leaf clipping. At 5 days post-inoculation, the inoculated leaves were assayed. a, Leaves were taken and analyzed for bacterial numbers and GUS activity was measured with the fluorogenic substrate 4-methylumbelliferyl-β-D-glucuronide. GUS activity values per 108 bacterial cells are the mean ± standard deviations of three independent measurements. b, GUS activity was measured using an in situ staining method, and bacterial cell numbers inside the infected leaves were measured in a parallel experiment. Average bacterial numbers inside the tested leaves are indicated. The experiments were repeated twice. Data presented are from a representative experiment, and similar results were obtained in the other independent experiment