Fig. 9

Competition between labelled wild-type and their prophage-cured derivative strains. a Ratio between labelled TIFN1 and TI1c cells propagated in mixed cultures in four different combinations and monitored by flow cytometry during 5 days. Green bars – GFP, red bars – mCherry, WT – wild type, c – cured derivative, G – GFP, Ch – mCherry. b Exemplary dot plots of experimental specimens shown in panel a recorded on propagation day 2 with encompassing gates for cell population expressing GFP-G, mCherry-R, negative cells-N and corresponding statistics; GFP-WT & mCherry-cured (upper panel) and GFP-cured & mCherry-WT (lower panel). On the y-axis is the GFP fluorescence intensity, on the x-axis – mCherry fluorescence intensity (log-scales). c Ratio between the GFP and mCherry expressing TIFN1 and TI1c cells after 2 days of propagation in mixed cultures as revealed by fluorescent colony count. d Exemplary images (overlay, pseudo colour) of the experimental specimens shown in panel c. GFP-WT & mCherry-cured (upper panel) and GFP-cured & mCherry-WT (lower panel). e Ratio between GFP-TI2c and mCherry-TIFN2 grown in mixed culture monitored by flow cytometry during 5 h