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Fig. 1 | BMC Microbiology

Fig. 1

From: Global gene-expression profiles of intracellular survival of the BruAb2_1031 gene mutated Brucella abortus in professional phagocytes, RAW 264.7 cells

Fig. 1

The levels of intracellular B. abortus wild-type and mutant strains in infected RAW 264.7 cells. RAW 264.7 cells were infected with wild-type and each mutant strain for 1 h at MOI 100, after which a gentamicin protection assay was conducted. At selected time points, the medium was removed and cells were washed prior to lysis, after which the lysate was plated on to brucella agar. a Internalization levels (0 h) of B. abortus wild-type and mutant strains in RAW 264.7 cells after gentamicin protection assay. b Intracellular CFU level at 6 h after internalization, indicating the levels of intracellular survival. c Intracellular CFU level of each strain at selected time points after internalization, indicating the levels of intracellular survival (6 h) and replication (12 h, 24 h, and 48 h) after internalization in RAW 264.7 cells. The CFU levels of (b) and (c) were expressed as a percentage compared to the respective CFU of internalization of each strain. (*p < 0.05 and **p < 0.01)

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