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Fig. 4 | BMC Microbiology

Fig. 4

From: Construction of Lactobacillus casei ghosts by Holin-mediated inactivation and the potential as a safe and effective vehicle for the delivery of DNA vaccines

Fig. 4

Localization of loaded DNA in L. Casei ghosts visualized by confocal laser scanning microscopy. a L. casei 393 ghosts filled with SYBR Green I labeled pCI-EGFP under white light. b L. casei ghosts filled with SYBR Green I labeled pCI-EGFP. Overlay of differential interference contrast and fluorescent image. The image indicated that the plasmid was filled within the bacterial ghosts. c Ghost membranes were stained with MitoTracker Green FM (green). d pCI-EGFP was detected by in situ hybridization with Cy3-labeled probes (red) specific for the EGFP. e All fluorescence photomicrographs (c, d) were taken of one middle z-scan section through the middle plane of the loaded bacterial ghosts and subsequently overlaid (e). Direct overlays of red and green fluorescent structures are represented by the yellow color

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BMC Microbiology

ISSN: 1471-2180

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