Fig. 2

16S rRNA gene-based phylogenetic tree showing evolutionary relationships of the 24 actinobacterial isolates possessing antimicrobial activity with their closest known taxa. The evolutionary history was inferred using the Neighbor-Joining method. The tree was constructed based on the evolutionary distances calculated using Kimura 2-parameter substitution model. All positions containing gaps and missing data were eliminated. The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test (1000 replicates) are shown next to the branches for values > 40%. The bar represents 0.02 substitutions per nucleotide position (2% sequence divergence). GenBank accession numbers are mentioned within the parentheses. Cryptobacterium curtum was the outgroup in the analysis. ^T = Type strain