Fig. 3
Chromatographic separation of proteins from yeast samples. The sample corresponding to the ammonium sulfate fraction with the highest antifreeze activity from L. creatinivorum (dotted line), C. parapsilosis (discontinuous line) and G. gastrica (continuous line) was loaded onto a Superdex 75 10/300 GL column. The mobile phase consisted of 20 mM sodium phosphate buffer at pH 7.0 and 150 mM NaCl at 0.2 ml min−1. A, SDS-PAGE analysis of fractions numbered from 1 to 5 (indicated in the corresponding chromatogram) from G. gastrica. B, Analysis of protein glycosylation: E, original extract; LP, low-molecular-weight proteins; HP, high-molecular-weight proteins; P, positive control; N, negative control. M, protein marker