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Table 3 Fragmentation patterns of signals at m/z 904.5, m/z 905.5, m/z 888.6 and m/z 882.6 obtained in the MS-spectra of Se°-nanoparticle samples and of particle sample extracts

From: Inhibition of bacteriochlorophyll biosynthesis in the purple phototrophic bacteria Rhodospirillumrubrum and Rhodobacter capsulatus grown in the presence of a toxic concentration of selenite

Analytes Precursor ion m/z Product ions     
   1st 2nd 3rd 4th
   m/z Intensity (% max.) m/z Intensity (% max.) m/z Intensity (% max.) m/z Intensity (% max.)
BChl ap Reference 910.5 632.2 100
BChl ag Particle samples R. rubrum 904.5 632.2 100 572.2 6.4 499.2 12.2
Bphe ap Reference 888.6 610.3 100 549.3 28.1 537.3 11.1 477.3 14.1
Bphe ap Particle sample extracts Rba. capsulatus 888.6 610.3 100 549.3 22.1 537.3 16.7 477.3 14.3
Bphe ap. Particle sample extracts R. rubrum 888.6 610.3 100 550.3 15.5 537.3 12.6 477.3 10.9
Bphe ag. Particle samples R. rubrum 882.6 610.3 100 551.3 12.1 537.3 16.9 477.3 13.6
Bphe ag. Particle sample extracts R. rubrum 882.6 610.3 100 549.3 16.8 537.3 12.0 477.3 14.7
  1. Tandem MS-spectra of MS-signals obtained for particle samples or particle sample extracts were compared with those of the references BChl ap and BPhe ap. Note that in each MS/MS-spectrum the most intense signal was for the loss of a 278.3 m. u. fragment or of a 272.3 m. u. fragment. As the mass of these fragments exactly corresponded to that of the phytyl side chain or the geranylgeranyl side chain, respectively (Fig. 2), these results confirmed that each of these MS-signals represented a BChl (BChl ap or BChl ag) or a BPhe (BPhe ap or BPhe ag) molecule.
  2. *Identified according to the mass values obtained in the MS-spectra