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Fig. 1 | BMC Microbiology

Fig. 1

From: Characterizing interactions of Leptospira interrogans with proximal renal tubule epithelial cells

Fig. 1

Virulent low passage (LP) strain colonizes the kidney, while high passage strain (HP) is cleared during hematogenous dissemination. a LP or HP strain were used to infect 5 weeks-old C57BL/6 mice. A sublethal dose of exponential growing bacteria was inoculated intraperitoneally and the presence of leptospiral DNA in the kidneys was monitored by qPCR at 1, 3, 10 and 25 days pi. b Immunofluorescence staining of LP- and HP-infected kidney at 25 days pi. Bacteria were detected with FITC-labeled secondary antibodies (green), actin were labeled with rhodamine-phalloidin (red) and DNA with TO-PRO-3 (blue). The merged images are also shown. c Histological features of kidneys from LP or HP-infected mice were compared with non-infected kidney at 25 days pi by hematoxylin and eosin staining

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