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Table 2 Biofilm formation on polystyrene microtiter plates, stainless steel coupons, glass slides and pellicle formation at the air-liquid interface

From: Correction to: Characterization of biofilm-forming capacity and resistance to sanitizers of a range of E.coli O26 pathotypes from clinical cases and cattle in Australia

Pathotypes

Isolates No.

Biofilm mass on polystyrene at incubation time of

Biofilm mass onb

Pellicle formationc

24 h

48 h

72 h

SS

GS

EHEC

EC1A

0.024 ± 0.004a

0.076 ± 0.015

0.049 ± 0.007

1

1

0.061 ± 0.011

EC1113B

0.029 ± 0.005

0.119 ± 0.022

0.191 ± 0.039

3

3

1.884 ± 0.259

EC1643B

0.047 ± 0.006

0.251 ± 0.033

1.126 ± 0.153

3

3

3.595 ± 0.191

EC1857

0.036 ± 0.006

0.063 ± 0.011

0.107 ± 0.016

3

3

0.856 ± 0.078

EC217

0.054 ± 0.009

0.100 ± 0.015

0.043 ± 0.008

2

1

0.025 ± 0.009

EC3455

0.066 ± 0.008

0.025 ± 0.005

0.060 ± 0.007

3

3

1.228 ± 0.145

EC3522

0.060 ± 0.008

0.058 ± 0.010

0.086 ± 0.011

1

1

0.039 ± 0.034

EC3547A

0.043 ± 0.003

0.236 ± 0.014

0.503 ± 0.030

2

2

0.839 ± 0.113

EC3652B

0.056 ± 0.010

0.102 ± 0.014

0.104 ± 0.016

3

3

1.875 ± 0.103

EC3659B

0.026 ± 0.009

0.058 ± 0.009

0.093 ± 0.014

3

3

1.652 ± 0.152

EC3671A

0.016 ± 0.005

0.091 ± 0.010

0.125 ± 0.017

3

3

1.554 ± 0.167

EC3738B

−0.010 ± 0.003

0.032 ± 0.013

0.093 ± 0.027

1

1

0.145 ± 0.030

EC3743A

0.038 ± 0.006

0.087 ± 0.011

0.075 ± 0.010

3

3

1.218 ± 0.087

EC4

0.023 ± 0.004

0.045 ± 0.009

0.024 ± 0.005

2

1

0.097 ± 0.023

EC478B

0.086 ± 0.011

0.127 ± 0.011

0.133 ± 0.011

2

2

0.480 ± 0.061

EC674

0.011 ± 0.005

0.064 ± 0.013

0.130 ± 0.023

3

3

1.660 ± 0.151

EC7B

0.082 ± 0.014

0.109 ± 0.013

0.230 ± 0.052

3

3

1.397 ± 0.091

EC4158QH1

0.047 ± 0.005

0.082 ± 0.015

0.069 ± 0.011

1

1

0.092 ± 0.042

EC4159QH2

0.049 ± 0.007

0.089 ± 0.013

0.055 ± 0.007

2

1

0.035 ± 0.025

EC4160QH3

0.098 ± 0.015

0.034 ± 0.008

0.044 ± 0.008

2

3

2.257 ± 0.099

EC3213QH34

0.036 ± 0.006

0.072 ± 0.007

0.171 ± 0.017

3

3

1.079 ± 0.046

EC4161QH4

0.075 ± 0.012

0.060 ± 0.010

0.061 ± 0.009

2

1

0.050 ± 0.021

EC4162QH5

0.001 ± 0.009

0.088 ± 0.020

1.021 ± 0.154

3

3

2.882 ± 0.255

EC4163QH6

0.017 ± 0.003

0.135 ± 0.030

1.469 ± 0.304

2

3

1.848 ± 0.127

EC4164QH7

0.053 ± 0.006

0.223 ± 0.025

0.245 ± 0.025

2

1

0.092 ± 0.034

EC4165QH8

0.070 ± 0.008

0.127 ± 0.019

0.156 ± 0.026

1

1

0.065 ± 0.037

EC4166QH9

0.061 ± 0.007

0.051 ± 0.010

0.070 ± 0.010

3

3

2.746 ± 0.163

pEHEC

EC801

0.040 ± 0.005

0.119 ± 0.017

0.121 ± 0.020

2

1

0.150 ± 0.042

EC3983A

0.033 ± 0.006

0.057 ± 0.011

0.074 ± 0.010

2

1

0.081 ± 0.024

EC3989A

0.063 ± 0.008

0.638 ± 0.036

1.402 ± 0.203

3

3

3.859 ± 0.138

aEPEC

EC3435A

0.044 ± 0.009

0.041 ± 0.007

0.060 ± 0.007

0

1

0.104 ± 0.064

EC3457

0.110 ± 0.020

0.134 ± 0.023

0.135 ± 0.014

1

1

−0.001 ± 0.030

EC3610A

0.019 ± 0.003

0.017 ± 0.004

0.033 ± 0.005

1

0

0.018 ± 0.023

EC3727A

0.037 ± 0.005

0.034 ± 0.007

0.119 ± 0.021

0

1

0.268 ± 0.051

EC3735A

0.046 ± 0.011

0.017 ± 0.011

0.108 ± 0.019

0

1

0.135 ± 0.027

EC3768A

0.035 ± 0.012

0.078 ± 0.017

0.206 ± 0.028

0

1

0.128 ± 0.020

EC4013A

0.048 ± 0.018

0.027 ± 0.011

0.101 ± 0.014

0

1

0.177 ± 0.032

EC4039A

0.006 ± 0.003

0.060 ± 0.009

0.048 ± 0.007

1

0

−0.005 ± 0.013

NTEC

EC3536B

0.052 ± 0.005

0.224 ± 0.021

0.475 ± 0.034

3

3

0.966 ± 0.158

EC3946A

0.052 ± 0.006

0.313 ± 0.056

0.404 ± 0.034

3

3

0.587 ± 0.127

Negative control

Lb broth

0.080 ± 0.0009

0.078 ± 0.001

0.071 ± 0.0004

0

0

 
  1. aValues are shown as mean of biofilm production ± standard error on polystyrene surfaces, SS: stainless steel, GS: glass slide at 25 °C. According to the biofilm
  2. mass quantified with crystal violet staining assay at 570 nm isolates were labelled as the following: low, medium and thick biofilm formers
  3. bVisible biofilms on stainless steel and glass slides and was scored as 0: no visible biofilm, scored on a scale from 1 to 3 to a thick biofilm at the air-liquid
  4. cThe presence and absence of visible pellicles biofilms was scored visually before staining with CV. Strains with visible pellicles at the air-liquid interface had OD570 ≥0.48
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BMC Microbiology

ISSN: 1471-2180

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