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Fig. 2 | BMC Microbiology

Fig. 2

From: Modelling invasive group A streptococcal disease using bioluminescence

Fig. 2

In vitro stability of transformed bioluminescent isolates: M1pLux, M3pLux, M89pLux and M89::Lux. Transformed strains a M1pLux b M3pLux c M89pLux d M89::Lux were passaged daily in THB with kanamycin (blue line) and without kanamycin (dashed blue line) to determine stability of the replicative plasmid pLux in vitro and the integrated plasmid pICL::lux. There was a significant reduction in the number of viable bacteria retaining the plasmid when passaged without kanamycin in comparison to the number of bacteria retaining the plasmid with kanamycin. Stability was determined by serial plating onto selective solid media. Median and range of three replicates per time point are shown

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