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Table 3 Primers used in this study

From: Transmating: conjugative transfer of a new broad host range expression vector to various Bacillus species using a single protocol

Primer Name

Sequence 5′ – 3′

pUB110_backbone_fw

GTCTAAGAAACCATTATTATCATGAC

pUB110_backbone_rv

CATAATAGTTATGCAGTTTGTAGAATGC

Fw_pUC_for_Gibson

CAAACTGCATAACTATTATGTACGAGCAAAAGGCCAGC

Rv_pUC_from_pBE-S

GTCAGGTGGCACTTTTCG

oriT_traJ_fw

TCCCCGAAAAGTGCCACCTGACTTAGTGCTTTACGGCACCTCG

oriT_traJ_rv

CATGATAATAATGGTTTCTTAGACGCTTCGGGGTCATTATAGCG

sfGFP-fw

TAAGCAAAAGGAGAGGGACGCGTATGAGCAAAGGTGAAGAACTG

sfGFP-rv

CATTAGTGGTGATGATGGTGATGTCTAGATTTATACAGTTCATCCATACCATG

pBACOV-seq_4894fw

CGAGTCTCTACGGAAATAGC

pBACOV-Seq-rv

CGATGAGCGCATTGTTAG

QC_g2015t_1

GCGGCGGCGGCAGGCATGAGCCTG

QC_g2015t_2

CAGGCTCATGCCTGCCGCCGCCGC