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Fig. 2 | BMC Microbiology

Fig. 2

From: Transmating: conjugative transfer of a new broad host range expression vector to various Bacillus species using a single protocol

Fig. 2

Schematic map of the shuttle vector pBACOV with the inserted target gene sfGFP. ColE1 ori: origin of replication for E. coli; AmpR: Ampicillin resistance gene for selection in E. coli; oriT/traJ: origin of transfer for conjugative plasmid transfer; KanR: Kanamycin resistance gene for selection in Bacillus; pUB ori: origin of replication for Bacillus; PaprE: aprE promoter from B. subtilis; sfGFP-His 6 : target gene sfGFP fused with a sequence encoding a C-terminal His6-tag

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BMC Microbiology

ISSN: 1471-2180

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