Characterisation of STEC and other diarrheic E. coli isolated on CHROMagar™STEC at a tertiary referral hospital, Cape Town

Table 1 Growth and Virulence properties of diarrhoeic E. coli that formed Mauve colonies on CHROMagar™STEC

Isolate No.	HUS	Bloody diarrhoea	Serotype	Virulence genes.	Pathotype	Immunoassay	TSB enrichment	Sorbitol
271		–	O55	eae,bfp	Typical EPEC		–	–
724		–	O55	eae,bfp	Typical EPEC		+	–
722.1		–	O55	eae,bfp	Typical EPEC		+	–
279		–	O55	eae, bfp	Typical EPEC		–	–
344		–	O55	eae, bfp	Typical EPEC		+	–
345.1		–	O55	eae, bfp	Typical EPEC		+	+
291		–	O182	eae	Atypical EPEC		–	+
286		–	O111	eae	Atypical EPEC		+	+
600		+	Not Identified	eae	Atypical EPEC		+	+
19		+	O26	eae	Atypical EPEC		–	–
284		–	O25	eae	Atypical EPEC		–	+
15.2		+	O26	eae	Atypical EPEC		–	–
63.2		–	O1	daaC	DAEC		–	+
689		–	O175	aat	EAggEC		+	–
326		–	O16	aat	EAggEC		+	–
231		–	O104	aat	EAggEC		–	–
229.1		–	O104	aat	EAggEC		–	–
473		–	O33	aat	EAggEC		+	+
371.1		+	O25	aat	EAggEC		+	–
336		–	O175	aat	EAggEC		+	+
229		–	O104	aat	EAggEC		–	–
733		–	O104	aat	EAggEC		+	–
502		–	O9	aat	EAggEC		+	+
688		–	O3	aat	EAggEC		+	+
480		–	O8	aat	EAggEC		+	+
250		–	O175	aat	EAggEC		–	+
207		–	O104	aat	EAggEC		–	+
696		–	O175	aat	EAggEC		+	+
598	–	–	O186	stx ₁ , eae	STEC	–	+	+
29.5	–	–	O186	eae, stx ₁	STEC	–	+	+
602	–	–	O101	eae, stx ₁ , stx ₂	STEC	–	+	–
232.1	–	–	Not identified	stx ₁	STEC	Shiga toxin 1	+	+
73		–	Not identified	stx ₁	STEC	Shiga toxin 1	+

EAggEC-enteroaggregative E. coli, STEC-Shiga-toxin producing E. coli, EPEC-enteropathogenic E. coli, DAEC-diffusely adherent E. coli. Fimbrial adhesion gene (daaC), the anti-aggregation protein transporter gene (aat), heat-stable (ST) and heat-labile (LT) enterotoxin genes, the intimin coding gene (eae) and the bundle-forming pili gene (bfp), Shiga toxin 1 (stx₁) and 2 (stx₂)

ISSN: 1471-2180