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Table 2 Quantitative biodegradation of caffeine by A. sydowii and A. niger on 5, 10 and 15th day

From: Biodegradation of caffeine by whole cells of tea-derived fungi Aspergillus sydowii, Aspergillus niger and optimization for caffeine degradation

Reaction time (d)

Fungal dry mass (g)

Cacaffeine (mg/L)

Ca theophylline (mg/L)

% of caffeine degraded b

A. sydowii NRRL250 (600 mg/L of caffeine)

 5

0.23 ± 0.02

431.5 ± 39.7

40.4 ± 1.0

28.1 ± 6.6

 10

0.24 ± 0.02

134.8 ± 6.5

209.9 ± 22.6

77.5 ± 1.1

 15

0.22 ± 0.01

3.7 ± 0.8

262.6 ± 20.7

99.4 ± 0.1

A. niger NCBT110A (600 mg/L of caffeine)

 5

0.28 ± 0.03

592.6 ± 3.1

NF

1.2 ± 0.5

 10

0.29 ± 0.02

580.0 ± 2.9

NF

3.3 ± 0.5

 15

0.27 ± 0.01

577.3 ± 6.0

NF

3.8 ± 1.0

  1. aC Concentration determined by HPLC
  2. b% of caffeine degraded was estimated as follow: of caffeine degraded = (C0-Ct)/C0*100% (1)
  3. In Eq. (1) C0 was the initial caffeine concentration (mg/L), Ct was the final caffeine concentration (mg/L) after the fermentation
  4. All data are presented as mean ± SD. NF not found