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Table 1 Primer sequences of macrophage genes used for qRT-PCR assays

From: Heat-stress-modulated induction of NF-κB leads to brucellacidal pro-inflammatory defense against Brucella abortus infection in murine macrophages and in a mouse model

Gene name Forward primer Reverse primer
b-actin 5’-CGCCACCAGTTCGCCATGGA-3′ 5’-TACAGCCCGGGGAGCATCGT-3
Il1b 5’-CAACCACACAAGTGATATTCTC-3′ 5’-GGATCCACACTCTCCAGCTGC-3
Il6 5’-TCCAGTTGCCTTCTTGGGAC-3’ 5’-GTACTCCAGAAGACCAGAG-3’
Tnf 5’-CACAGAAAGCATGATCCGCGA-3’ 5’-CGGCAGAGAGGAGGTTGACTT-3’
Il10 5’-TGGCCCAGAAATCAAGGAGC-3’ 5’-CAGCAGACTCAATACACACT-3’
Ifng 5’-TGAACGCTACACACTGCATCT-3’ 5’-CGACTCCTTTTCCGCTTCCTG-3’
Mcp1 5′- GGTCCCTGTCATGCTTCTGGG-3’ 5’-TCCAGCCTACTCATTGGGATC-3’
P50 5’-CTCACTCAATATTTAATGCAG-3’ 5’-CCCTCCGTGTGATGGGCCTTC-3’
P52 5’-CCCATGGAGGTTTGCCAGGTG-3’ 5’-CCCACCAGACTGTGGGCATGC-3’
P65 5’-CATCCACATGAACTTGTGGGG-3’ 5’-CTGGCTAATGGCTTGCTCCAG − 3’