A Recurrent Silent Mutation Implicates fecA in Ethanol Tolerance by Escherichia coli

Lupino, Katherine M.; Romano, Kymberleigh A.; Simons, Matthew J.; Gregg, John T.; Panepinto, Leanna; Cruz, Ghislaine M.; Grajek, Lauren; Caputo, Gregory A.; Hickman, Mark J.; Hecht, Gregory B.

doi:10.1186/s12866-018-1180-1

BMC Microbiology

Table 5 Mutations observed in ethanol tolerant strains derived from FBR5^a

From: A Recurrent Silent Mutation Implicates fecA in Ethanol Tolerance by Escherichia coli

	Locus			High Permeability Strains^b	Low Permeability Strains^c
Functional Category	Gene	Function	Mutation	High MIC	High MIC	Low MIC
Membrane Structure and Function
	xylG	Component of a D-xylose ABC transporter	G67R	ARM*
	wza	Outer membrane polysaccharide exporter	insertion at ~ 2,134,249 nt		ARS*
	waaG	Catalyzes formation of D-glycosyl-lipopolysaccharide	S359R			ANE, ANF
	yebZ	Presumed inner membrane protein with unknown function	G164 fs			ANE, ANF
	wbbH	O antigen assembly	V97E			ARQ*
	ynfB	Presumed periplasmic protein, unknown function	V34 M			ANC
	rybB	sRNA regulating degradation of omp mRNAs in response to envelope stress^d	P4T			ANC, AND
Ion Transport and Usage
	fecA	Ferric citrate import^e	V244 V	ARN, ARO*	ANG*	ANI*
	yhiD	Active transport of Mg²⁺ into the cell	T170A	ARN
	yfeX	Iron retrieval from heme and PPIX	E151A			ANC, AND
Carbon Metabolism
	tdcE	Pyruvate formate lyase	N26D	ARL*
	cdaR	D-galactarate and D-glucarate metabolism	L151Q		ARK
	fdhF	Formate dehydrogenase	V87F			ANA*
	yfdE	Oxalate CoA-transferase	K317 T			ANE, ANF
Respiration
	intergenic between torS/torT	TMAO reductase	Insertion		ANJ
	ndh	NADH deydrogenase II^f	R306R			AND
Protein Synthesis and Related Functions
	valT,lysW,valZ	tRNA genes	779,852–780,370 deletion	ARN
	rrlC	Encodes 23S rRNA	Insertion at nt 3,943,588		ANJ
Pyrimidine Metabolism
	carB	Carbamoyl phosphate synthase	W213S	ARP
	preT	Pyrimidine degradation	S32 T	ART
	ygeW	Carbamoyl-transferase	D289V		ANJ
Miscellaneous
	gyrA	Gyrase; nalidixic acid resistance	R739C	ARN
	rhsD	Inhibits growth of neighboring bacteria	T524A	ART
	rhsA	Inhibits growth of neighboring bacteria	Insertion at nt 3,762,076 and deletion at nt 3,763,391		ANJ
	ytcA	hypothetical protein	A49T		ANJ
	cheA	Histidine kinase regulating flagellar rotation (chemotaxis)	P618L		ARK
	many genes	deletion	excision of e14 prophage^g			ANE, ANF
No mutations detected in ORFs					ARR	ANB, ANH

^aTable lists mutations detected in open reading frames; for full list of all observed mutations, please see Additional File 1: Table S1. Abbreviations and symbols: fs, framsehsift; MIC, minimal inhibitory concentration; nt, nucleotide position; ORF, open reading frame; PPIX, protoporphyrin IX; TMAO, trimethylamine-N-oxide; *indicates strains for which only a single nucleotide alteration from FBR5 was observed
^bThese mutants display constitutively higher inner membrane permeability than FBR5 (see text and Table 3)
^cThese mutants display inner membrane permeability that is either similar to FBR5 or is higher than FBR5 but not constitutively (see text and Table 3)
^dVarious omp genes were implicated in ethanol stress response in [28]
^ePreviously implicated in ethanol stress response [28]
^fPreviously implicated in ethanol tolerance in [29]
^gSee refs. [69, 70]

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ISSN: 1471-2180

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