A Recurrent Silent Mutation Implicates fecA in Ethanol Tolerance by Escherichia coli

BMC Microbiology

Table 1 E. coli strains and plasmids used in this study

Strain or plasmid	Genotype or relevant characteristics^a	Source or reference
E. coli K-12
BW25113	MG1655 derivative; F⁻, Δ(araD-araB)567 ΔlacZ4787(::rrnB-3) λ⁻ rph-1 Δ(rhaD-rhaB)568 hsdR514	[15]
BW25113 Δ JW4251	BW25113 ΔfecA758::kan	[66]
FBR5	∆pfl::Cm ldhA::kan, pLOI297	[4]
MG1655	F⁻, λ⁻ ilvG⁻ rfb-50 rph-1	K-12 reference strain; ATCC47076
Plasmids
pCA24N	Cm^r vector, IPTG-inducible promoter	[67]
pCA24N + JW4251	pCA24N derivative carrying fecA gene adjacent to IPTG-inducible promoter	[67]
pLOI297	Ap^r Tc^r pdc⁺ adhB⁺	[68]

^aAbbreviations: Ap ampicillin, Cm chloramphenicol, Kan kanamycin, Tc tetracycline

ISSN: 1471-2180