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Fig. 2 | BMC Microbiology

Fig. 2

From: A Recurrent Silent Mutation Implicates fecA in Ethanol Tolerance by Escherichia coli

Fig. 2

MIC experiments were conducted using serial dilutions to demonstrate acquired ethanol tolerance. Mutants were considered to have a high MIC if they were capable of showing growth above an OD of 0.1 at 600 nm at 4.5% EtOH. Strains unable to grown to above an OD of 0.1 at 600 nm at 4.5% EtOH were determined to have a low MIC. All strains, FBR5 and the 20 isolated mutants, grew to an OD under 0.1 in 9% EtOH and to an OD of ≥0.1 in 2.25% EtOH (results not shown). Data shown represents the results from at least three independent trials. The error bars represent one standard deviation above and below the mean of the data. Mutant strains are presented in the order of their MIC phenotypes and 30 °C ONPG permeability (see also Fig. 3 and Table 3). Blue columns represent data from strains categorized as “High MIC” strains and red columns represent data from strains categorized as “Low MIC” strains

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