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Fig. 5 | BMC Microbiology

Fig. 5

From: Characterising resuscitation promoting factor fluorescent-fusions in mycobacteria

Fig. 5

Functionality of Rpf-mCherry/EGFP fusions: nutritional shift-down assay. The assay was performed as detailed in Methods. a Rpf-mCherry fusions are functional: their overproduction stimulates the growth of stationary cells subjected to nutritional shift-down much faster than the overproduction of mCherry alone. Growth curves were made with data collected from 3 biological replicates. Error bars indicate standard error of the mean. b Rpf-EGFP fusions are not functional: their overproduction does not stimulate the growth of cells subjected to nutritional shift-down any better than the negative control overproducing EGFP. A representative growth curve (of at least 3 biological replicates) is shown for each strain

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